One milliliter of blood from a healthy human donor was filtered to deplete red blood cells by size selection. Then, 15,000 cells in 1 mL of cell media was loaded into each HIVE collector. Three cell-loaded HIVEs were processed immediately and three were stored at -20C for two weeks before processing. The HIVEs were processed following the standard protocol, and the resulting libraries were sequenced on an Illumina NovaSeq. BeeNet v.1.1.0 with hg38 was used to analyze this dataset.
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